The evening twice and then at night. The lines were super dark and the answer was quick. I then took one at the doctor's office in the morning a couple days later.

middle of the day/ evening. even the cheap dollar test picked up 14-16 days after conception without first morning urine.

The first morning pee is what your supposed to go by. If you are pregnant that's when the HcG hormone is the highest.

I would take it both time. But probably when you wake up the second time would be better since you'll go a longer time without drinking water that dilutes the hormones in your pee. lol. @tristynn

@marfie15, I get up in the middle of the night and go to the restroom. Would I take it when I get up at night or just wait until I wake up a second time?

All times. lol. With my first I took it in the afternoon then one in the morning. Did the same thing with this one, but also took one late at night. It's best to test in the morning tho because I got faint positives later in the day and it leaves more room for a fake negative

I took mine at 1 in the morning

noon and again at about 6pm

took all of mine at night they showed up instantly